What level should I aim for?

Most physicians target 40–60 ng/mL — comfortably in the adequate range with a buffer.

How long does treatment take?

An 8-week high-dose course (60,000 IU once a week) usually restores levels, followed by a maintenance dose.

How often should I retest?

3 months after starting supplements, then annually if stable.

20 minutes of midday sun on arms and face, 3–4 times a week, helps — but most office-going Indians still come up deficient. Supplements are often needed.

Why is the test so expensive?

It uses a specialised technique (chemiluminescence). We've priced it 33% below MRP at ₹1,200.

PathologyHome collection

Vitamin D 25-Hydroxy

Also called: 25(OH)D, Vitamin D Test, Vitamin D3

Measures stored Vitamin D — the most accurate way to check for deficiency.

Duration: 5 min
Report: 48 hours
Sample: Blood
Fasting: Not needed

₹1,800₹1,20033% OFFSave ₹600

NABL-accreditedExpert team4.8 ★ avg rating

Save more — get this in the Comprehensive Health Checkup

13 tests · 62 parameters · ₹2,499

What this test is

25-Hydroxy Vitamin D is the form your body stores and the best single indicator of your Vitamin D status. Deficiency is extremely common across urban India even in sunny cities — most adults working indoors test low. Levels under 20 ng/mL are deficient; 20–30 are insufficient; 30–60 are adequate. Symptoms of deficiency include bone pain, muscle weakness, frequent infections, and low mood. Supplementation is cheap and effective once levels are confirmed.

When you need this test

Bone or muscle pain
Frequent infections or low immunity
Persistent fatigue or low mood
Indoor-job adults — annual screening
Pregnancy
Pre-menopausal and post-menopausal women
Children with poor growth

How to prepare

No fasting needed.
If you take Vitamin D supplements, mention your dose and how long you've been on it.
Avoid biotin supplements for 48 hours — they can interfere.

Why DiagnoHouse for this test

NABL-accredited lab
Quality-audited blood, urine, and stool analysis.
Home collection
Free across Pimpri-Chinchwad. Sample picked up at your time slot.
Same-day reports
Most pathology reports ready within 4 hours of collection.
Calibrated equipment
Analysers calibrated daily; quality controls run with every batch.

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FAQs

What level should I aim for?
Most physicians target 40–60 ng/mL — comfortably in the adequate range with a buffer.
How long does treatment take?
An 8-week high-dose course (60,000 IU once a week) usually restores levels, followed by a maintenance dose.
How often should I retest?
3 months after starting supplements, then annually if stable.
Is sunlight enough?
20 minutes of midday sun on arms and face, 3–4 times a week, helps — but most office-going Indians still come up deficient. Supplements are often needed.
Why is the test so expensive?
It uses a specialised technique (chemiluminescence). We've priced it 33% below MRP at ₹1,200.

Need help choosing?

Not sure if this is the right test for you? Have a doctor's note? Call or WhatsApp — guidance is free.

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5,000+ scans · 4.8 ★ avg rating · NABL accredited · Reports on WhatsApp